Human/Mouse Wnt-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4751
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 55025
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-4
Ile37-Glu76
Accession # NP_033549
Ile37-Glu76
Accession # NP_033549
Specificity
This antibody detects human and mouse Wnt-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Wnt-1, 2b, 5a, 5b, 8a, 8b, 9b, 10a, 10b, 11, 16, recombinant human (rh) Wnt-2, 3a, 7a, or 7b is observed. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep
Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF6076).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse Wnt-4 Antibody
Detection of Human Wnt‑4 by Western Blot.
Western blot shows lysates of human breast cancer tissue. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Wnt‑4 in MCF‑7 Human Cell Line.
Wnt-4 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Wnt-4 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and SK-BR-3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Wnt-4 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Applications for Human/Mouse Wnt-4 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody for human Wnt-4 when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
2 µg/mL
Sample: Human breast cancer tissue, MCF‑7 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Human breast cancer tissue, MCF‑7 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-4
Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Alternate Names
Wnt4
Gene Symbol
WNT4
UniProt
Additional Wnt-4 Products
Product Documents for Human/Mouse Wnt-4 Antibody
Product Specific Notices for Human/Mouse Wnt-4 Antibody
For research use only
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