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Human MS4A4A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7797

R&D Systems, part of Bio-Techne
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MAB7797
MAB7797-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 818112

Product Specifications

Immunogen

Mouse myeloma cell line NS0 transfected with human MS4A4A
Accession # Q96JQ5

Specificity

Detects human MS4A4A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MS4A4A Antibody

Detection of MS4A4A antibody in HEK293 Human Cell Line Co-transfected with MS4A4A and eGFP antibody by Flow Cytometry.

Detection of MS4A4A in HEK293 Human Cell Line Co-transfected with MS4A4A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line co-transfected with human MS4A4A and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human MS4A4A Monoclonal Antibody (Catalog # MAB7797) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human MS4A4A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µL/106 cells
Sample: Human MS4A4A and eGFP co-transfectants

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MS4A4A

MS4A4A, also called CD20-like 1, is a 23 kDa (predicted) member of the MS4A family of four-transmembrane proteins that includes CD20 (MS4A1) and Fce RIb (MS4A2). MS4A4 cDNA is detected in hematopoietic cell lines, with highest expression in myeloid and B cell lines. Expressed sequence tags are detected in multiple organs and tissues. Human MS4A4A shares ~60% amino acid sequence identity with mouse and rat MS4A4A, if sequence gaps at the cytoplasmic N and C termini are disregarded. One potential isoform lacks aa 130-182, which includes the 3rd transmembrane segment and the first of two extracellular regions.

Long Name

Membrane-spanning 4-domains, Subfamily A, Member 4A

Alternate Names

4SPAN1, CD20L1, MS4A4, MS4A7

Entrez Gene IDs

51338 (Human); 666907 (Mouse); 361734 (Rat)

Gene Symbol

MS4A4A

UniProt

Additional MS4A4A Products

Product Documents for Human MS4A4A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MS4A4A Antibody

For research use only

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