Human MUC-4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8195A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 781631

View all MUC-4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MUC-4
Pro1072-Ser1317
Accession # Q99102

Specificity

Detects human MUC-4 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MUC-4 APC-conjugated Antibody

Detection of MUC-4 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of MUC-4 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-4 APC-conjugated Monoclonal Antibody (Catalog # FAB8195A, filled histogram) or isotype control antibody (IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MUC-4 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MUC-4

MUC-4 (Mucin-4), also called ASGP (ascites sialoglycoprotein) is a highly glycosylated type I transmembrane glycoprotein that may be up to 950 kDa in its full-length, fully glycosylated form. Human MUC-4 cDNA encodes 2169 amino acids (aa) with a 28 aa signal sequence and a cleavage site that creates a 1416 aa soluble, extracellular alpha chain and a 725 aa single-pass transmembrane beta chain. Between aa 1072-1317 within the alpha chain, human MUC-4 shares 69% aa sequence identity with mouse and rat MUC-4. At least 14 soluble or transmembrane splice variants of 1102-2117 aa have been described, 5 of which contain the full sequence used as an immunogen. MUC-4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC-4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Entrez Gene IDs

4585 (Human); 140474 (Mouse)

Gene Symbol

MUC4

UniProt

Q99102

Additional MUC-4 Products

Product Documents for Human MUC-4 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MUC-4 APC-conjugated Antibody

For research use only

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