Human MuSK Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3904
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MuSK
Glu22-Ser503
Accession # O15146
Glu22-Ser503
Accession # O15146
Specificity
Detects human MuSK in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant rat MuSK is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MuSK Antibody
Detection of Mouse MuSK by Western Blot
Sorbs1 is enriched at AChR aggregates, and Sorbs1 RNAi blocks AChR clustering in vitro. (A) Treatment of myotubes with siRNA directed against Sorbs1 blocks AChR clustering in C2C12 cells. Montages containing sixteen fields at a magnification of 10× were analyzed with ImageJ software (NIH). (B) Sorbs1 siRNA significantly reduces Sorbs1 protein expression in myotubes. (C) Sorbs1 protein is highly enriched at sites where AChRs aggregate. (D) Agrin stimulates tyrosine phosphorylation of MuSK and Dok-7 at similar levels in myotubes treated with Sorbs1 siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26527617), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human MuSK Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MuSK
Sample: Recombinant Human MuSK
Reviewed Applications
Read 2 reviews rated 3 using AF3904 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MuSK
MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22‑503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail.
Long Name
Muscle-specific Receptor Tyrosine Kinase
Alternate Names
EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase
Gene Symbol
MUSK
UniProt
Additional MuSK Products
Product Documents for Human MuSK Antibody
Product Specific Notices for Human MuSK Antibody
For research use only
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