Human MyD88 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29281
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 316628
Product Specifications
Immunogen
E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836
Met1-Pro296
Accession # Q99836
Specificity
Detects human MyD88 in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse MyD88 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human MyD88 Antibody
Detection of MyD88 in Raji Human Cell Line by Flow Cytometry.
Raji human Burkitt's lymphoma cell line was stained with Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB29281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin.Applications for Human MyD88 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Raji human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: Raji human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MyD88
Myeloid Differentiation primary response gene 88 (MyD88) contains a cytoplasmic Toll/interleukin 1 receptor (TIR) and functions as an adaptor protein in Toll-like receptor signaling. It is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.
Long Name
Myeloid Differentiation Primary Response Gene 88
Alternate Names
MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88
Gene Symbol
MYD88
UniProt
Additional MyD88 Products
Product Documents for Human MyD88 Antibody
Product Specific Notices for Human MyD88 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...