Human NAALADase-2/NAALAD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7658
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 756030
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-2/NAALAD2
Lys32-Leu740
Accession # Q9Y3Q0
Lys32-Leu740
Accession # Q9Y3Q0
Specificity
Detects human NAALADase-2/NAALAD2 in direct ELISAs.
In direct ELISAs, approximately 50%
cross-reactivity with recombinant mouse NAALADase-2 is observed and no
cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like
2, or rhNAALADase-like 1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NAALADase-2/NAALAD2 Antibody
Detection of Human NAALADase-2/NAALAD2 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NAALADase-2/NAALAD2 in Human Testis.
NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/ NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NAALADase-2/NAALAD2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Western Blot
0.5 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NAALADase-2/NAALAD2
Long Name
N-Acetylated Alpha-Linked Acidic Dipeptidase 2
Alternate Names
GCPIII, Glutamate Carboxypeptidase III, NAALAD2, NAALADase2
Gene Symbol
NAALAD2
UniProt
Additional NAALADase-2/NAALAD2 Products
Product Documents for Human NAALADase-2/NAALAD2 Antibody
Product Specific Notices for Human NAALADase-2/NAALAD2 Antibody
For research use only
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