LNCaP human prostate cancer cell line was stained with Mouse Anti-Human NAALADase-like 2/NAALADL2 Monoclonal Antibody (Catalog # MAB4665, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human NAALADase-like 2/NAALADL2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: LNCaP human prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha-linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAALADL2 shares 87% and 82% aa sequence identity with mouse and canine NAALADL2, respectively.

Long Name

N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2

Alternate Names

NAALADase like 2, NAALADL2

Entrez Gene IDs

254827 (Human); 635702 (Mouse); 499583 (Rat)

Gene Symbol

NAALADL2

UniProt

Q58DX5

Additional NAALADase-like 2/NAALADL2 Products

Product Documents for Human NAALADase-like 2/NAALADL2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human NAALADase-like 2/NAALADL2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NAALADase-like 2/NAALADL2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4665

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NAALADase-like 2/NAALADL2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NAALADase-like 2/NAALADL2 Antibody

Detection of NAALADase‑like 2/ NAALADL2 in LNCaP Human Cell Line by Flow Cytometry.