Human Neprilysin-2/MMEL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2340
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 353207
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2
Gly78-Trp779
Accession # Q495T6
Gly78-Trp779
Accession # Q495T6
Specificity
Detects human Neprilysin‑2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Neprilysin, rhECE‑1 or rhECE‑2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Neprilysin-2/MMEL1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)
Sample: Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)
Reviewed Applications
Read 1 review rated 4 using MAB2340 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neprilysin-2/MMEL1
Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles.
References
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.
Alternate Names
MMEL1, MMEL2, Neprilysin2, NL2, SEP
Entrez Gene IDs
79258 (Human)
Gene Symbol
MMEL1
UniProt
Additional Neprilysin-2/MMEL1 Products
Product Documents for Human Neprilysin-2/MMEL1 Antibody
Product Specific Notices for Human Neprilysin-2/MMEL1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...