Human Neudesin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6714
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 737130
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neudesin
Gly32-Phe172
Accession # Q9UMX5
Gly32-Phe172
Accession # Q9UMX5
Specificity
Detects human Neudesin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Neudesin Antibody
Detection of Human Neudesin by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, IMR-32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neudesin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neudesin in Human Brain.
Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Neudesin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line, IMR‑32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line
Sample: MCF‑7 human breast cancer cell line, IMR‑32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neudesin
Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human Neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human Neudesin shares 97% aa identity with mature mouse Neudesin
Alternate Names
CIR2, NENF, SCIRP10, SPUF
Gene Symbol
NENF
UniProt
Additional Neudesin Products
Product Documents for Human Neudesin Antibody
Product Specific Notices for Human Neudesin Antibody
For research use only
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