Human Neuromedin BR/NMBR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4728
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Met390
Accession # AAB27330
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Neuromedin BR/NMBR Antibody
Detection of Neuromedin B R/ NMBR in HT‑29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Detection of Neuromedin B R/NMBR in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) Neuromedin B R/NMBR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.Applications for Human Neuromedin BR/NMBR Antibody
CyTOF-ready
Flow Cytometry
Sample: HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP
Intracellular Staining by Flow Cytometry
Sample: HT-29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuromedin BR/NMBR
Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity. Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Neuromedin BR/NMBR Products
Product Documents for Human Neuromedin BR/NMBR Antibody
Product Specific Notices for Human Neuromedin BR/NMBR Antibody
For research use only