Human NFATC3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5834

R&D Systems, part of Bio-Techne
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AF5834
AF5834-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all NFATC3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968

Specificity

Detects human NFATC3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NFATC3 Antibody

Detection of Human and Rat NFATC3 antibody by Western Blot.

Detection of Human NFATC3 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human NFATC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC3 at approximately 145 - 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NFATC3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC3

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145‑150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109‑114), two nuclear localization sites (aa 273‑275 and 686‑688), one nuclear export site (aa 1032‑1041) and an RH domain that binds DNA (aa 433‑592). There are multiple isoforms with different tissue expression profiles. Over aa 900‑1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Long Name

Nuclear Factor of Activated T Cells C3

Alternate Names

NFAT4, NFATX

Entrez Gene IDs

4775 (Human); 18021 (Mouse)

Gene Symbol

NFATC3

UniProt

Q12968

Additional NFATC3 Products

Product Documents for Human NFATC3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NFATC3 Antibody

For research use only

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