Human NFkB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2697
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 285412
Product Specifications
Immunogen
E. coli-derived recombinant human NF kappaB1
Pro317-Asp437
Accession # P19838
Pro317-Asp437
Accession # P19838
Specificity
Detect endogenous human NF kappaB1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NFkB1 Antibody
Detection of Human NF kappaB1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse NF kappa B1 Monoclonal Antibody (Catalog # MAB2697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF kappa B1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human NFkB1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NFkB1
Nuclear Factor kappaB1 (NF kappaB1 or NF kappaB p50) is a member of the NF kappaB/Rel family of transcription factors. NF kappaB1 dimerizes with other members of the NF kappaB/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.
Long Name
Nuclear Factor Kappa-B, Subunit 1
Alternate Names
NF-kB1
Gene Symbol
NFKB1
UniProt
Additional NFkB1 Products
Product Documents for Human NFkB1 Antibody
Product Specific Notices for Human NFkB1 Antibody
For research use only
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