Human NGL-3/LRRC4B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6919
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 702021
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human NGL-3/LRRC4B
Ala47-Lys576
Accession # Q9NT99
Ala47-Lys576
Accession # Q9NT99
Specificity
Detects human NGL-3/LRRC4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human LRRC3, 4, or 32 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NGL-3/LRRC4B Antibody
NGL‑3/LRRC4B in Human Brain.
NGL-3/LRRC4B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human NGL-3/ LRRC4B Monoclonal Antibody (Catalog # MAB6919) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human NGL-3/LRRC4B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NGL-3/LRRC4B
Long Name
Netrin-G3 Ligand/Leucine-rich Repeat Containing 4B
Alternate Names
LRIG4, LRRC4B, NGL3
Gene Symbol
LRRC4B
UniProt
Additional NGL-3/LRRC4B Products
Product Documents for Human NGL-3/LRRC4B Antibody
Product Specific Notices for Human NGL-3/LRRC4B Antibody
For research use only
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