Human Nicotinamide N-Methyltransferase/NNMT Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7736
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 954208
Product Summary for Human Nicotinamide N-Methyltransferase/NNMT Antibody
Immunogen
E.coli-derived recombinant human Nicotinamide N‑Methyltransferase/NNMT
Met1-Leu264
Accession # P40261
Met1-Leu264
Accession # P40261
Specificity
Detects human Nicotinamide N‑Methyltransferase/NNMT in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Nicotinamide N-Methyltransferase/NNMT Antibody
Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot.
Western blot shows lysates of human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nicotinamide N-Methyltransferase/NNMT Monoclonal Antibody (Catalog # MAB7736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nicotinamide N-Methyltransferase/NNMT at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Nicotinamide N-Methyltransferase/NNMT Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786‑O human renal cell adenocarcinoma cell line
Sample: Human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786‑O human renal cell adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nicotinamide N-Methyltransferase/NNMT
Long Name
Nicotinamide N-Methyltransferase
Alternate Names
Nicotinamide NMethyltransferase, NNMT
Entrez Gene IDs
4837 (Human)
Gene Symbol
NNMT
UniProt
Additional Nicotinamide N-Methyltransferase/NNMT Products
Product Documents for Human Nicotinamide N-Methyltransferase/NNMT Antibody
Product Specific Notices for Human Nicotinamide N-Methyltransferase/NNMT Antibody
For research use only
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