Human NLRP2/NALP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4684
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 443625
Product Specifications
Immunogen
E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02
Met1-Thr208
Accession # Q9NX02
Specificity
Detects human NLRP2/NALP2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human NLRP2/NALP2 Antibody
Detection of Human NLRP2/NALP2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NLRP2/NALP2 Monoclonal Antibody (Catalog # MAB4684) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human NLRP2/NALP2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line
Reviewed Applications
Read 1 review rated 3 using MAB4684 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP2/NALP2
NLRP2, also known as NALP2, is a cytosolic 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappaB, it suppresses TNF‑ alpha production in response to LPS; via procaspase‑1, it promotes proIL‑1 beta cleavage and release. Human NLRP2 is 1062 amino acids (aa) in length. It contains an N‑terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa 109‑132, aa 133‑154 and aa 847‑1062, respectively; a fourth shows a 38 aa substitution for aa 1‑903. No close rodent counterparts have been reported.
Long Name
NLR Family, Pyrin Domain Containing 2
Alternate Names
NALP2, NBS1, PAN1, PYPAF2
Gene Symbol
NLRP2
UniProt
Additional NLRP2/NALP2 Products
Product Documents for Human NLRP2/NALP2 Antibody
Product Specific Notices for Human NLRP2/NALP2 Antibody
For research use only
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