HEK293 cells transfected with Human NPR2 and eGFP were stained with either (A) Mouse Anti-Human NPRB/NPR2 Monoclonal Antibody (Catalog # MAB9725) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NPRB/NPR2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 cells transfected with Human NPR2 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRB/NPR2

Human Natriuretic Peptide Receptor-2 (NPR2), also known as NPRB, ANP-RB or guanylyl Cyclase-B, is a member of the guanylyl cyclase family of proteins. NPR2 is a type I transmembrane glycoprotein that contains a 436 amino acid extracellular domain (ECD) (aa 23‑458) for ligand binding, and a 569 amino acid cytoplasmic domain that contains both a protein kinase domain and a carboxyl-terminal guanylate cyclase domain. NPR2 is expressed most highly in in bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue (1). NPR2 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B type natriuretic peptide), and NPR2 is the principal receptor of CNP (C type natriuretic peptide) (1, 2). Ligand binding to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (2). NPR2 pathway play a critical role in regulation of skeletal growth (3), and patients with single defect NPR2 alleles are statistically shorter than the average population (4). Over the extracellular domain, human NPR2 is 97% and 96% identical to mouse and rat NPR2, respectively.

References

Potter, L.R. et al. (2009) Handb Exp Pharmacol 191:341.
Chang, M.S. et al. (1989) Nature 341:68.
Tsuji, T. and Kunieda T. (2005) J Biol Chem 280:14288.
Olney, R.C. et al. (2006) J Clin Endocrinol Metab 91:1229.

Long Name

Natriuretic Peptide Receptor B

Alternate Names

AMDM, ANPb, ANPRB, ECDM, GC-B, GUC2B, NPR2, NPRBi, SNSK

Entrez Gene IDs

4882 (Human); 230103 (Mouse); 116564 (Rat)

Gene Symbol

NPR2

UniProt

P20594

Additional NPRB/NPR2 Products

Product Documents for Human NPRB/NPR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NPRB/NPR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human NPRB/NPR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9725

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NPRB/NPR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NPRB/NPR2 Antibody

Detection of NPRB/NPR2 in HEK293 cells transfected with Human NPR2 and eGFP by Flow Cytometry.