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Human NPY1R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6400

R&D Systems, part of Bio-Techne
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MAB6400
MAB6400-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-reported, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 556153

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human NPY1R
Met1-Ile384
Accession # P25929

Specificity

Detects human NPY1R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NPY1R Antibody

Detection of NPY1R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line (Korner, M and J.C. Reubi (2007) Peptides28:419) was stained with Human NPY1R Monoclonal Antibody (Catalog # MAB6400, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human NPY1R Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPY1R

Neuropeptide Y receptor type 1 (NPY1R) is a 44 kDa (unglycosylated) member of the G protein coupled receptor 1 family. Human NPY1R is 384 amino acids (aa) in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa 368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for neuropeptide Y and peptide YY.

Long Name

Neuropeptide Y Receptor Y1

Alternate Names

NPYY1

Entrez Gene IDs

4886 (Human); 18166 (Mouse)

Gene Symbol

NPY1R

UniProt

Additional NPY1R Products

Product Documents for Human NPY1R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NPY1R Antibody

For research use only

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