Human NTB-A/SLAMF6 APC-conjugated Antibody

Detection of NTB-A/SLAMF6 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB-A/SLAMF6 APC-conjugated Monoclonal Antibody (Catalog # FAB19081A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human NTB-A/SLAMF6/CD352 by Flow Cytometry

Functional activity of Rev1-Vpu expressed from pCG expression plasmids.(A) CMV-IE promoter-based pCG expression vectors containing vpu (left panel) or the rev1-vpu fusion gene (right panel). An enhanced version of the green fluorescent protein (eGFP) is co-expressed via an IRES. (B) Expression of Rev1-Vpu and Vpu in HEK293T cells transfected with the indicated pCG vectors. A Vpu-specific antiserum was used for detection. eGFP was detected to check transfection efficiencies. (C-F) FACS analysis of (C) CD4, (D) tetherin, (E) CD1d or (F) NTB-A receptor modulation by ZM247 Vpu and Rev1-Vpu. HEK293T cells were transfected with expression vectors for the respective surface receptor and Vpu or Rev1-Vpu. 40 h post transfection, surface receptor levels were monitored by two-color flow cytometry. Dot plots indicating the gating strategy are shown in the right panels. Bar diagrams summarizing three to five independent experiments +/- SD are shown on the left (***p<0.001; **p<0.01; *p<0.05; n.s. not significant). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0142118), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human NTB-A/SLAMF6/CD352 by Flow Cytometry

Functional characterization of Vpus from Cameroonian HIV-1 N strains.(A) FACS analysis of 293T cells cotransfected with tetherin, CD4, CD1d or NTB-A expression vectors and pCGCG plasmids expressing eGFP alone (lanes 2 and 3) or together with the indicated vpu allele (lanes 4 to 9). eGFP expression levels used to calculate receptor downmodulation and the mean fluorescence intensities (MFIs) are indicated. (B) Effect of various Vpus on infectious virus release. 293T cells were cotransfected with HIV-1 deltaVpu NL4-3 (2 µg), pCGCG vectors coexpressing eGFP and Vpu (500 ng), and a construct expressing HU tetherin (125 ng). Viral supernatants were obtained two days later and used to measure the quantity of infectious HIV-1 in the culture supernatants by infecting TZM-bl indicator cells. Shown is the infectious virion yield relative to that obtained in the absence of tetherin (100%). The results were confirmed in two independent experiments and each symbol indicates infectious virus yield in the presence of one of the 25 vpu alleles analyzed. (C–F) Vpu-dependent reduction of (C) tetherin, (D) CD4, (E) CD1d and (F) NTB-A surface expression in 293T cells. Shown is the reduction in the levels of receptor cell surface expression relative to those measured in cells transfected with the eGFP only control vector. Each symbol represents n-fold downmodulation of the indicated receptor molecule by one individual vpu allele examined. Shown are average values derived from three experiments. Vpu alleles derived from HIV-1 M are color coded red, N blue (except for the DJO0131 Vpu highlighted in green) and SIVcpz black; those shown in panel A are indicated by open symbols in panels B to F. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23308067), licensed under a CC-BY license. Not internally tested by R&D Systems.