Human ORF73 HHV8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10389
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # LN53
Product Specifications
Immunogen
Recombinant protein corresponding to the latent nuclear antigen-1 molecule of ORF73 HHV8
Specificity
Detects human ORF73 HHV8 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human ORF73 HHV8 Antibody
ORF73 HHV8 in Kaposi's Sarcoma Skin Tissue.
ORF73 HHV8 was detected in immersion fixed paraffin-embedded sections of Kaposi's Sarcoma skin tissue using Mouse Anti-Human ORF73 HHV8 Monoclonal Antibody (Catalog # MAB10389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human ORF73 HHV8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Kaposi's Sarcoma skin tissue
Sample: Immersion fixed paraffin-embedded sections of Kaposi's Sarcoma skin tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ORF73 HHV8
Lana1 (also known as ORF73 or HHV8) is a multifunctional protein that plays a role in the replication and long-term persistence of the viral episomal genome in dividing cells. Lana1 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for Lana1 in kaposi's sarcoma.
Long Name
ORF73 of Human Herpesvirus 8
Alternate Names
HHV8GK18_gp81, LANA1
Entrez Gene IDs
4961527 (Viral)
Gene Symbol
HHV8GK18_GP81
Additional ORF73 HHV8 Products
Product Documents for Human ORF73 HHV8 Antibody
Product Specific Notices for Human ORF73 HHV8 Antibody
For research use only
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