Hek293 cells transfected with Human P2RX7 peptide and eGFP were stained with either (A) Mouse Anti-Human P2X7/P2RX7 Monoclonal Antibody (Catalog # MAB97903) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human P2X7/P2RX7 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Hek 293 cells transfected with Human P2RX7 peptide and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2X7/P2RX7

Human P2X purinoceptor 7 (aka P2X7) is a 595 aminoacids (aa) protein encoded by the P2RX7 gene. P2X7 receptors belong to the family of ATP-gated ion channels, and their activity can be found in cells of hemopoietic lineage including macrophages, microglia, and certain lymphocytes. They mediate the influx of Ca2+ and Na+ and the release of pro-inflammatory cytokines. At least 8 different human P2X7 isoforms formed by alternative splicing have been reported, ranging from 128 aa to 595 aa. The P2X7 subunits can form homomeric receptors only with a typical P2X receptor structure.

Long Name

P2X Purinergic Receptor 7

Alternate Names

P2RX7

Entrez Gene IDs

5027 (Human); 18439 (Mouse); 29665 (Rat)

Gene Symbol

P2RX7

UniProt

Q99572

Additional P2X7/P2RX7 Products

Product Documents for Human P2X7/P2RX7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human P2X7/P2RX7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human P2X7/P2RX7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB97903

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all P2X7/P2RX7 Antibodies »

Product Summary for Human P2X7/P2RX7 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human P2X7/P2RX7 Antibody

Detection of P2X7/P2RX7 in Hek 293 cells transfected with Human P2RX7 peptide and eGFP by Flow Cytometry.