Human Pax3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2457
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Avian - Chicken
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pax3
Met1-Ser215
Accession # NP_000429
Met1-Ser215
Accession # NP_000429
Specificity
Detects Pax3 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5 and 1% rhPax7, under reducing conditions, is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Pax3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed B16-F1 mouse melanoma cell line
Sample: Immersion fixed B16-F1 mouse melanoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Pax3
Sample: Recombinant Human Pax3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax3
Long Name
Paired Box Gene 3 [Waardenburg Syndrome 1]
Alternate Names
HUP2, WS1
Gene Symbol
PAX3
UniProt
Additional Pax3 Products
Product Documents for Human Pax3 Antibody
Product Specific Notices for Human Pax3 Antibody
For research use only
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