A549 human lung carcinoma cell line was stained with Mouse anti-Human PDGFRL Monoclonal Antibody (Catalog # MAB1723, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human PDGFRL Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGFRL

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.

References

Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
Matsui, T. et al. (1989) Science 243:800.
Hou, S. et al. (2013). Human Mutat. 34:74.
Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Alternate Names

PDGFR-Like Protein, PDGRL, PRLTS

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Gene Symbol

PDGFRL

UniProt

Q15198

Additional PDGFRL Products

Product Documents for Human PDGFRL Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PDGFRL Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PDGFRL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1723

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PDGFRL Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PDGFRL Antibody

Detection of PDGFRL in A549 Human Cell Line by Flow Cytometry