Human Peptide YY Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB94562
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1011014
Product Specifications
Immunogen
Human Peptide YY synthetic peptide
Specificity
Detects human Peptide YY in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Peptide YY Antibody
Human Peptide YY ELISA Standard Curve.
Recombinant Human Peptide YY protein was serially diluted 2-fold and captured by Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Peptide YY Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94561).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peptide YY
Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.
Alternate Names
Peptide-YY, PYY, PYY-I, PYY1
Gene Symbol
PYY
Additional Peptide YY Products
Product Documents for Human Peptide YY Antibody
Product Specific Notices for Human Peptide YY Antibody
For research use only
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