Human Peptide YY Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB94563
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Applications
Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2581A
Product Summary for Human Peptide YY Antibody
Immunogen
Human Peptide YY synthetic peptide
Accession # P10082
Accession # P10082
Specificity
Detects Peptide YY in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Peptide YY Antibody
Detection of Human Peptide YY by Simple WesternTM.
Simple Western lane view shows lysates of human colon tissue and human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Peptide YY at approximately 3 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB94563) . This experiment was conducted under reducing conditions and using the 2-40. kDa separation system.Applications for Human Peptide YY Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Human colon tissue and Human small intestine tissue
Sample: Human colon tissue and Human small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peptide YY
Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.
Alternate Names
Peptide-YY, PYY, PYY-I, PYY1
Gene Symbol
PYY
UniProt
Additional Peptide YY Products
Product Documents for Human Peptide YY Antibody
Product Specific Notices for Human Peptide YY Antibody
For research use only
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