Human Phospho-CEP250/CNAP1 (S2421) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11339

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB11339-100
MAB11339-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2739C

View all CNAP1/CEP250 Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human CEP250/CNAP1 S2421 site
Accession # Q9BV73

Specificity

Detects Human Phospho-CEP250/CNAP1 in direct ELISA 

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-CEP250/CNAP1 (S2421) Antibody

Detection of Human CNAP1/CEP250 by Western Blot.

Western blot shows lysates of MDA-MB-231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CNAP1/CEP250 Monoclonal Antibody (Catalog # MAB11339) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CNAP1/CEP250 at approximately ~250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Phospho-CEP250/CNAP1 (S2421) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MDA-MB-231 human breast cancer cells and MDA-MB-231 + Calyculin A

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNAP1/CEP250

CEP250 (also known as CNAP1) is a large centrosome-associated protein that plays a crucial role in centrosome duplication and organization during cell division. Centrosomes are organelles play an essential role in cell division, cell polarity, and intracellular transport. In most animal cells, centrosomes consist of a pair of centrioles surrounded by pericentriolar material (PCM).CEP250/CNAP1 is a critical component of the PCM and is required for centrosome duplication and maturation. It forms a bridge between the centrioles and PCM and recruits other PCM components to the centrosome. Phosphorylation of CEP250/CNAP1 may regulate association/dissociation from centrosome. During M phase of mitosis, it is phosphorylated by NEK2, which may trigger the dissociation from the mitotic centrosome. This antibody detects CEP250/CNAP1 phosphorylated at Ser2421.  

Long Name

Centrosomal Nek2-Associated Protein 1

Alternate Names

C-NAP1, CEP2, CEP250

Entrez Gene IDs

11190 (Human)

Gene Symbol

CEP250

UniProt

Q9BV73

Additional CNAP1/CEP250 Products

Product Documents for Human Phospho-CEP250/CNAP1 (S2421) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-CEP250/CNAP1 (S2421) Antibody

For research use only

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