Human Phospho-eIF4B (S422) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3800
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 866049
Product Specifications
Immunogen
Phosphopeptide containing the human eIF4B S422 site
Specificity
Detects human eIF4B when phosphorylated at S422
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-eIF4B (S422) Antibody
Detection of Human Phospho-eIF4B (S422) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes or 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF4B (S422) Monoclonal Antibody (Catalog # MAB3800) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF4B (S422) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-eIF4B (S422) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with 200 nM PMA or Recombinant Human EGF (Catalog # 236-EG)
Sample: HeLa human cervical epithelial carcinoma cell line treated with 200 nM PMA or Recombinant Human EGF (Catalog # 236-EG)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF4B
eIF4B (eukaryotic translation initiation factor 4B), an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with the heterotrimeric eIF4F, stimulates the ATPase and RNA helicase activity of eIF4E.
Long Name
Eukaryotic Translation Initiation Factor 4B
Alternate Names
Eif4a2
Gene Symbol
EIF4B
Additional eIF4B Products
Product Documents for Human Phospho-eIF4B (S422) Antibody
Product Specific Notices for Human Phospho-eIF4B (S422) Antibody
For research use only
Loading...
Loading...
Loading...
Loading...