Human Phospho-MSK1(S376)/MSK2(S360) Antibody

Detection of Human MSK1/MSK2 by Western Blot

Phosphorylation changes after treatment for different ES and OS cell lines.(A) Cytarabine’s effect on STA-ET.7.2 cells compared to the clofarabine treatment. Cells were treated with clofarabine, cytarabine or DMSO. Immunoblot analysis showed cytarabine did not alter phosphorylation of ERK1/2, MSK1/2 and CREB; (B) Immunoblot results of phosphorylation changes after clofarabine treatment of different ES cell lines; clofarabine caused increased phosphorylation of ERK1/2, MSK1/2 and CREB in all five ES cell lines; (C) Clofarabine treated OS cell lines did not show significant increase in phosphorylation of ERK1/2, MSK1/2 and CREB. Actin was used as the loading control for all experiments. (CLF: Clofarabine, CYT: Cytarabine). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34133426), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human MSK1/MSK2 by Western Blot

Clofarabine and CD99 antibodies cause specific protein phosphorylation in ES cell lines.(A) STA-ET-7.2 cells treated with clofarabine and western blot analyses were done by phosphospecific antibodies. Phosphorylation of ERK1/2, MSK1/2 and CREB levels were increased with clofarabine treatment; (B) TC-71 cells treated with two different C99 antibodies (15μg/ml) and phosphorylation changes were analyzed by immunoblotting. Similar to clofarabine treatment, phosphorylation of ERK1/2, MSK1/2 and CREB were increased; (C, D) STA-ET-7.2 cells treated with clofarabine, CD 99 antibody and decreased phosphorylation changes for STAT3 and AMPK alpha1 were validated by western blotting; (E) A4573 cells treated with clofarabine show increased level of pMSK1/2 and main phosphorylated protein is pMSK2. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34133426), licensed under a CC-BY license. Not internally tested by R&D Systems.