Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1094
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human MSK1 S376 site
Specificity
Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively. The amino acid sequence surrounding S360 of mouse MSK2 is identical to the phosphopeptide antigen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody
Detection of Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: HEK293 human embryonic kidney cell line treated with Recombinant Human EGF (Catalog # 236-EG)
Sample: HEK293 human embryonic kidney cell line treated with Recombinant Human EGF (Catalog # 236-EG)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1/MSK2
Long Name
Mitogen- and Stress-activated Protein Kinase 1 and 2
Alternate Names
1110069D02Rik, 3110005L17Rik, 6330404E13Rik
Additional MSK1/MSK2 Products
Product Documents for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody
Product Specific Notices for Human Phospho-MSK1 (S376)/MSK2 (S360) Antibody
For research use only
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