Human Phospho-Rad17 (S635) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1374
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1244A
Product Specifications
Immunogen
Phosphopeptide containing the human Rad17 (S635)
Accession # O75943
Accession # O75943
Specificity
Detects human Rad17 when phosphorylated at S635 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-Rad17 (S635) Antibody
Detection of Human Phospho-Rad17 (S635) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 25 µM Etoposide for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Rad17 (S635) Polyclonal Antibody (Catalog # MAB1374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Rad17 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-Rad17 (S635) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Etoposide
Sample: Jurkat human acute T cell leukemia cell line treated with Etoposide
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad17
The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).
Alternate Names
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog
Gene Symbol
RAD17
UniProt
Additional Rad17 Products
Product Documents for Human Phospho-Rad17 (S635) Antibody
Product Specific Notices for Human Phospho-Rad17 (S635) Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
Loading...
Loading...
Loading...
Loading...