Human Phospho-RPA2 (T21) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6654
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing the human RPA2 T21 site
Accession # P15927
Accession # P15927
Specificity
Detects human RPA2 when phosphorylated at T21.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-RPA2 (T21) Antibody
Detection of Human Phospho‑RPA2 (T21) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour. PVDF Membrane was probed with 0.5 µg/mL of Human Phospho-RPA2 (T21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6654) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RPA2 (T21) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-RPA2 (T21) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line treated with Camptothecin (CPT)
Sample: HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line treated with Camptothecin (CPT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RPA2
RPA2 (replication protein A 32 kDa subunit; also RFA2 and RPA p34) is a 32 kDa DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70 kDa RPA1 and 14 kDa RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270 amino acids (aa) in length. It contains a Gly/Ser-rich N-terminus (aa 1-33), a DNA-binding domain (aa 43-171) and a protein-interaction C-terminus (aa 187-270). Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N-terminal extensions: one shows an 88 aa insertion after Ser4, another shows a 12 aa substitution for aa 1-4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa 93-98, and a potential truncation after Gln175. Over aa 141-270, human RPA2 shares 83% aa identity with mouse RPA2.
Long Name
Replication Protein A2, 32kDa
Alternate Names
REPA2, RPA32
Gene Symbol
RPA2
UniProt
Additional RPA2 Products
Product Documents for Human Phospho-RPA2 (T21) Antibody
Product Specific Notices for Human Phospho-RPA2 (T21) Antibody
For research use only
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