Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-STAT1 (Y701) Monoclonal Antibody (Catalog # MAB2894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-STAT1 (Y701) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT1 (Y701) Molyclonal Antibody (Catalog # MAB2894) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human Phospho-STAT1 (Y701) Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: IFN alpha-treated Daudi Human Cell Line fixed with paraformaldehyde and permeabilized with methanol

Western Blot

0.25 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT1

STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human); 20846 (Mouse)

Gene Symbol

STAT1

UniProt

NP009330

Additional STAT1 Products

Product Documents for Human Phospho-STAT1 (Y701) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-STAT1 (Y701) Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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Flow Cytometry

Human Phospho-STAT1 (Y701) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2894

Key Product Details

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Product Specifications

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Scientific Data Images for Human Phospho-STAT1 (Y701) Antibody

Detection of Human Phospho-STAT1 (Y701) by Western Blot.