Human PKC zeta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6666
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 453513
Product Summary for Human PKC zeta Antibody
Immunogen
E. coli-derived recombinant human PKC zeta
Ile454-Val592
Accession # Q05513
Ile454-Val592
Accession # Q05513
Specificity
Detects human PKC zeta in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) PKC iota and rhPKC alpha and no cross-reactivity with rhPKC epsilon, eta, beta 1, beta 2, delta, gamma, nu, mu, or theta is observed. In Western blots, 100% cross-reactivity with recombinant human (rh) PKC iota and no cross-reactivity with rhPKC epsilon or eta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PKC zeta Antibody
Detection of Human PKC zeta by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKC zeta Monoclonal Antibody (Catalog # MAB6666) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKC zeta at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PKC zeta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using MAB6666 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC zeta
Long Name
Protein Kinase C zeta
Alternate Names
PKC2, PRKCZ
Gene Symbol
PRKCZ
UniProt
Additional PKC zeta Products
Product Documents for Human PKC zeta Antibody
Product Specific Notices for Human PKC zeta Antibody
For research use only
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