Human PLA2G2A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5374
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
CyTof
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 620501
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G2A
Asn21-Cys144
Accession # P14555
Asn21-Cys144
Accession # P14555
Specificity
Detects human PLA2G2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human PLA2G1B, G2A, or G7 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PLA2G2A Antibody
Detection of Human PLA2G2A by Western Blot.
Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Human PLA2G2A Monoclonal Antibody (Catalog # MAB5374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLA2G2A at approximately 17 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLA2G2A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G2A (Catalog # 4925-PL), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human PLA2G2A (Catalog # 4925-PL), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Human spleen tissue under non-reducing conditions only
Sample: Human spleen tissue under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLA2G2A
Phospholipase A2 Group IIA (PLA2G2A) is a 17 kDa member of the phospholipase A2 family of enzymes that hydrolyzes the sn-2 ester bond of phospholipids. Human PLA2G2A is synthesized as a 144 amino acid (aa) precursor that contains a 20 aa signal sequence and a 124 aa mature chain. Mature human PLA2G2A shares 72%, 68%, and 60% aa sequence identity with mature rat, mouse, and bovine PLA2G2A, respectively. PLA2G2A is a calcium dependent phospholipase expressed in many cell types associated with inflammation, including platelets, neutrophils, and mast cells. Most secretory PLA2s are stored in cytoplasmic granules and are released into the extracellular environment on appropriate cell activation. Thus, they are present at higher concentrations in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic diseases.
Long Name
Phospholipase A2 Group IIA
Alternate Names
Mom1, PLA2B, PLA2L, RASF-A
Gene Symbol
PLA2G2A
UniProt
Additional PLA2G2A Products
Product Documents for Human PLA2G2A Antibody
Product Specific Notices for Human PLA2G2A Antibody
For research use only
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