Human PLD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5615
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLD1
Met1-Pro140
Accession # Q13393
Met1-Pro140
Accession # Q13393
Specificity
Detects endogenous human PLD1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PLD1 Antibody
Detection of Human PLD1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PLD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5615) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLD1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLD1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and U937 human histiocytic lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLD1
PLD1 (Phospholipase D1a) is a 110‑120 kDa member of the phospholipase D family of enzymes. It is expressed in endothelial cells and select tissues. Following activation and association with Type I alpha PIPkinase, PLD1 hydrolyzes the phosphodiester bond of membrane phosphatidylcholine, generating phosphatidic acid. Human PLD1 is 1074 amino acids in length). It contains one PX/phox homology domain (aa 84‑206), a pleckstrin homology domain (aa 219‑328), and two phosphodiesterase enzyme regions (aa 459‑486 and 891‑918). There are three splice variants. One shows a 10 aa substitution for aa 962‑1074 (PLD1d), a second shows an 84 aa substitution for aa 514‑1074 (PLD1c), and a third shows an Asn substitution for aa 585‑623 (PLD1b). Caspase cleavage of PLD1a at Gln545‑Ser546 generates a 62 kDa N-terminal, and a 61 kDa C-terminal fragment. Over aa 1‑140, human PLD1a shares 91% aa identity with mouse PLD1a.
Long Name
Phospholipase D1
Alternate Names
Choline phosphatase 1
Gene Symbol
PLD1
UniProt
Additional PLD1 Products
Product Documents for Human PLD1 Antibody
Product Specific Notices for Human PLD1 Antibody
For research use only
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