Human PLK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4197
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLK3
Pro320-Ala520
Accession # Q9H4B4.2
Pro320-Ala520
Accession # Q9H4B4.2
Specificity
Detects human PLK3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PLK3 Antibody
Detection of Human PLK3 by Western Blot.
Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLK3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLK3
Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK1, PLK2, PLK3, and PLK4. PLK3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase delta, p125 on Ser60.
Long Name
Polo-like Kinase 3
Alternate Names
CNK, FNK, PRK
Gene Symbol
PLK3
UniProt
Additional PLK3 Products
Product Documents for Human PLK3 Antibody
Product Specific Notices for Human PLK3 Antibody
For research use only
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