Human Polypeptide GalNAc Transferase 1/GALNT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7140

R&D Systems, part of Bio-Techne
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AF7140
AF7140-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Polypeptide GalNAc Transferase 1/GALNT1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 1/GALNT1
Gly41-Phe559
Accession # Q10472

Specificity

Detects human Polypeptide GalNac Transferase 1/GALNT1 in direct ELISAs and Western blots. In direct ELISAs, less than5% cross‑reactivity with recombinant human (rh) GALNT2 and rhGALNT3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Polypeptide GalNAc Transferase 1/GALNT1 Antibody

Polypeptide GalNac Transferase 1/GALNT1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Polypeptide GalNac Transferase 1/GALNT1 in Human Liver.

Polypeptide GalNac Transferase 1/GALNT was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Polypeptide GalNac Transferase 1/GALNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7140) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Polypeptide GalNAc Transferase 1/GALNT1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.1 µg/mL
Sample: Recombinant Human Polypeptide GalNac Transferase 1/GALNT1 (Catalog # 7140-GT)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Polypeptide GalNAc Transferase 1/GALNT1

O-glycosylation is a ubiquitous post-translational modification of secreted and membrane-bound proteins. Polypeptide N‑acetylgalactosaminyl­transferases (GALNTs) calalyze the initial step for o‑glycosylation: transferring GalNAc to Thr or Ser residues (GalNAc  alpha1‑O‑Ser/Thr) in the Golgi compartment. Structurally, the GALNTs consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites (1, 2). Twenty distinct GALNT isoforms have been detected in humans. Most of the isoforms display both unique and overlapping substrate specificities (3, 4) with no universal consensus glycosylation sequence. Glycosylation of mucins results from successive, often hierarchical, action of several specific GALNTs (5). GALNT1, in particular, is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein (6). Using a peptide library screening approach, GALNT1 was classified as an early transferase that has a preference for nonglycosylated or monoglycosylated substrates (5). The enzymatic activity of recombinant human GALNT1 was determined using a phosphatase-coupled assay (7).

References

  1. Gerken, T.A. et al. (2011) J. Biol. Chem. 286:14493.
  2. Ten Hagen, K.G. et al. (2003) Glycobiology 13:1R.
  3. Hagen, F.K. et al. (1997) J. Biol. Chem. 272:13843.
  4. Gerken, T.A. et al. (2006) J. Biol. Chem. 281:32403.
  5. Pratt, M.R. et al. (2004) Chem. Biol. 11:1009.
  6. Miwa, H.E. et al. (2010) J. Biol. Chem. 285:1208.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1

Alternate Names

GALNAC-T1, pp-GaNTase 1

Entrez Gene IDs

2589 (Human); 14423 (Mouse); 79214 (Rat)

Gene Symbol

GALNT1

UniProt

Q10472

Additional Polypeptide GalNAc Transferase 1/GALNT1 Products

Product Documents for Human Polypeptide GalNAc Transferase 1/GALNT1 Antibody

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Product Specific Notices for Human Polypeptide GalNAc Transferase 1/GALNT1 Antibody

For research use only

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