Human POR/Cytochrome P450 Reductase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6340
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 739613
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human POR/Cytochrome P450 Reductase
Arg45-Ser677
Accession # P16435
Arg45-Ser677
Accession # P16435
Specificity
Detects human POR/Cytochrome P450 Reductase in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human POR/Cytochrome P450 Reductase Antibody
Detection of Human POR/Cytochrome P450 Reductase by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POR/Cytochrome P450 Reductase Monoclonal Antibody (Catalog # MAB6340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human POR/Cytochrome P450 Reductase Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POR/Cytochrome P450 Reductase
NADPH-Cytochrome P450 Reductase (POR, P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). POR is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. POR is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. POR is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of POR can result in disordered steroidogenesis and Antley-Bixler syndrome.
References
- Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
- Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
- Flueck C.E. et al. (2004) Nat. Genet. 36:228.
Long Name
NADPH Cytochrome P450 Reductase
Alternate Names
CPR, CYPOR, Cytochrome P450 Reductase, P450R
Entrez Gene IDs
5447 (Human)
Gene Symbol
POR
UniProt
Additional POR/Cytochrome P450 Reductase Products
Product Documents for Human POR/Cytochrome P450 Reductase Antibody
Product Specific Notices for Human POR/Cytochrome P450 Reductase Antibody
For research use only
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