Human PP4 Catalytic Subunit Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5074
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 501517
Product Specifications
Immunogen
E. coli-derived recombinant human PP4 Catalytic Subunit
Met1-Leu307
Accession # P60510
Met1-Leu307
Accession # P60510
Specificity
Detects human, mouse, and rat PP4 Catalytic Subunit in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PP4 Catalytic Subunit Antibody
Detection of Human PP4 Catalytic Subunit by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PP4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP4 Catalytic Subunit at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PP4 Catalytic Subunit Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP4
The catalytic subunit of Protein Phosphatase 4 (PP4), also called PPX, is a serine/threonine protein phosphatase with about 65% homology to PP2A. Although PP4 has the same heterotrimeric subunit structure as PP2A and can use many of the PP2A regulatory subunits, PP4 also has its own unique scaffolding and regulatory subunits. PP4 is largely found in the centrosome of the nucleus where it may play a role in modulating transcription factor phosphorylation by JNK. In Drosophila, cmm mutants have low levels of PP4 and are unable to complete mitosis due to a deficit in microtubule assembly.
Long Name
Protein Phosphatase 4
Alternate Names
PP4C, PPH3, PPP4C, PPX
Gene Symbol
PPP4C
UniProt
Additional PP4 Products
Product Documents for Human PP4 Catalytic Subunit Antibody
Product Specific Notices for Human PP4 Catalytic Subunit Antibody
For research use only
Loading...
Loading...
Loading...
Loading...