Human PPAR alpha/NR1C1 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H0723-00

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Direct ELISA, Gel Supershift Assay, Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H0723

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Recombinant human PPAR alpha/NR1C1
aa 4-96

Specificity

This antibody specifically recognizes human PPAR alpha and cross-reacts with mouse PPAR alpha. This antibody does not cross-react with human PPAR gamma or PPAR delta. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human PPAR alpha/NR1C1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions should be determined by each laboratory.

Direct ELISA

This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect human PPAR alpha.

Gel Supershift Assay

This antibody can be used at 100 μg/mL with the appropriate secondary reagents to detect human PPAR alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 2 μg/mL under reducing conditions with the appropriate secondary reagents to detect human PPAR alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha; NR1C1) is a member of the Nuclear Receptor superfamily. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three members have been identified and are called PPAR alpha, delta, and lambda. Retinoid X Receptor (RXR) is an obligate partner for PPAR.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

5465 (Human)

Gene Symbol

PPARA

Additional PPAR alpha/NR1C1 Products

Product Documents for Human PPAR alpha/NR1C1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PPAR alpha/NR1C1 Antibody

For research use only

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