Human PPP2R4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5929
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257
Glu14-Asn323
Accession # Q15257
Specificity
Detects human PPP2R4 in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PPP2R4 Antibody
Detection of Human PPP2R4 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, U266 human myeloma cell line, and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PPP2R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PPP2R4 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PPP2R4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line, U266 human myeloma cell line, and HDLM‑2 human Hodgkin's lymphoma cell line
Sample: MCF‑7 human breast cancer cell line, U266 human myeloma cell line, and HDLM‑2 human Hodgkin's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PPP2R4
Long Name
Serine/threonine Protein Phosphatase 2A Regulatory Subunit B
Alternate Names
PTPA
Gene Symbol
PTPA
UniProt
Additional PPP2R4 Products
Product Documents for Human PPP2R4 Antibody
Product Specific Notices for Human PPP2R4 Antibody
For research use only
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