Human PREB Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5557
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PREB
Met1-Ser388
Accession # Q9HCU5
Met1-Ser388
Accession # Q9HCU5
Specificity
Detects human PREB in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PREB Antibody
Detection of Human PREB by Western Blot.
Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human PREB Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human tonsil tissue
Sample: Human tonsil tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PREB
PREB (Prolactin regulatory element-binding protein; also mSEC12) is a 45 kDa member of the WD-repeat protein family. Although it is ubiquitously expressed, its expression level varies, tissue-to-tissue. It apparently acts as both a DNA-binding transcription factor, and a catalyst for vesicle formation from the ER. Human PREB is a 417 amino acid (aa) type II transmembrane protein. It is found in the ER and nuclear envelope, and may undergo splicing to generate a transcriptional factor. PREB contains a large cytoplasmic region (aa 1‑388) that displays three WD repeats (aa 152‑337), and an eight aa lumenal domain (aa 410‑417). The transcription factor region lies between aa 175‑417. Multiple splice forms exist. There is an alternate start site at Met73 that may be accompanied by either a 47 aa substitution for aa 388‑417, or a 14 aa substitution for aa 409‑417. Over aa 1‑388, human PREB shares 88% aa identity with mouse PREB.
Long Name
Prolactin Regulatory Element Binding
Alternate Names
MGC3467, SEC12
Gene Symbol
PREB
UniProt
Additional PREB Products
Product Documents for Human PREB Antibody
Product Specific Notices for Human PREB Antibody
For research use only
Loading...
Loading...
Loading...
Loading...