I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?

We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Human Progesterone R B/NR3C3 B Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H5344-00

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Direct ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # H5344

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Product Specifications

Immunogen

Recombinant human Progesterone R B/NR3C3
aa 2-99

Specificity

This antibody specifically reacts with human PR isoform B and cross-reacts with mouse and rat PR isoform B. It does not cross-react with human PR isoform A. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human Progesterone R B/NR3C3 B Antibody

Application

Recommended Usage

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human PR Isoform B.

Immunohistochemistry

This antibody can be used at 10 - 20 μg/mL with the appropriate secondary reagents to detect human PR Isoform B.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions and at 3 μg/mL under non-reducing conditions with the appropriate secondary reagents to detect human PR Isoform B.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Progesterone R B/NR3C3

Progesterone Receptor (PR; NR3C3) is a member of the Steroid Receptor superfamily. PR binds Progesterone. It is expressed in the uterus, ovary, vagina, fallopian tubes and breast. PR plays a central role in diverse reproductive events associated with the establishment and maintenance of pregnancy, alveolar development in the breast, and sexual behavior. Two isoforms of this receptor have been identified, PR-A and PR-B, 94 and 114 kDa in size, respectively. It has been shown that PR-A and PR-B isoforms can form homodimers as well as heterodimers.

Long Name

Progesterone Receptor B

Alternate Names

PGRB, Progesterone RB

Entrez Gene IDs

5241 (Human)

Gene Symbol

PGR

Additional Progesterone R B/NR3C3 Products

Product Documents for Human Progesterone R B/NR3C3 B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Progesterone R B/NR3C3 B Antibody

For research use only

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