Human PSMA/FOLH1/NAALADase I Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB42342
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 460407
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609
Lys44-Ala750
Accession # Q04609
Specificity
Detects Human PSMA/FOLH1/NAALADase I in direct ELISAs
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PSMA/FOLH1/NAALADase I Antibody
Detection of PSMA/FOLH1 in LNCaP Human Cell Line by Flow Cytometry.
LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1 Monoclonal Antibody (Catalog # MAB42342, filled histogram) or Mouse IgG2A isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human PSMA/FOLH1/NAALADase I Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: LNCaP human prostate cancer cell line
Sample: LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
References
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
- Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Alternate Names
FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I
Gene Symbol
FOLH1
UniProt
Additional PSMA/FOLH1/NAALADase I Products
Product Documents for Human PSMA/FOLH1/NAALADase I Antibody
Product Specific Notices for Human PSMA/FOLH1/NAALADase I Antibody
For research use only
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