Human PTGER3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10243
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 606609
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human PTGER3
Met1-Arg390
Accession # P43115
Met1-Arg390
Accession # P43115
Specificity
Detects human PTGER3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PTGER3 Antibody
Detection of Human PTGER3 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human PTGER3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTGER3 at approximately 73 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Cannabinoid PTGER3 in HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human PTGER3 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB10243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.Applications for Human PTGER3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human PTGER3 and eGFP
Western Blot
2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human PTGER3
Sample: HEK293T human embryonic kidney cell line transfected with human PTGER3
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTGER3
Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.
Long Name
Prostaglandin E2 Receptor 3, Subtype EP3
Alternate Names
EP3
Gene Symbol
PTGER3
UniProt
Additional PTGER3 Products
Product Documents for Human PTGER3 Antibody
Product Specific Notices for Human PTGER3 Antibody
For research use only
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