Human PTH1R/PTHR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB57091

R&D Systems, part of Bio-Techne
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MAB57091-100
MAB57091-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 542407

View all PTH1R/PTHR1 Antibodies »

Product Summary for Human PTH1R/PTHR1 Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PTHR1
Tyr23-Met189
Accession # Q03431

Specificity

Detects human PTHR1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PTH1R/PTHR1 Antibody

PTH1R/PTHR1 antibody in Human Kidney by Immunohistochemistry (IHC-P).

PTH1R/PTHR1 in Human Kidney.

PTH1R/PTHR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB57091) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PTH1R/PTHR1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTH1R/PTHR1

PTH1R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66-68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca++-release from bone, mediated by osteoclast formation. Mature human PTH1R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1R signaling. Over aa 1-189, human PTH1R shares 88% aa identity with mouse PTH1R.

Long Name

Parathyroid Hormone Receptor 1

Alternate Names

PTHR, PTHR1

Entrez Gene IDs

5745 (Human); 19228 (Mouse); 56813 (Rat)

Gene Symbol

PTH1R

UniProt

Q03431

Additional PTH1R/PTHR1 Products

Product Documents for Human PTH1R/PTHR1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTH1R/PTHR1 Antibody

For research use only

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