Human PTH1R/PTHR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5709
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 734630
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PTH1R/PTHR1
Tyr23-Met189
Accession # Q03431
Tyr23-Met189
Accession # Q03431
Specificity
Detects human PTH1R/PTHR1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PTH1R/PTHR1 Antibody
Detection of Human PTH1R/PTHR1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos-2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB5709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTH1R/PTHR1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PTH1R/PTHR1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
2 µg/mL
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos‑2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos‑2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTH1R/PTHR1
Long Name
Parathyroid Hormone Receptor 1
Alternate Names
PTHR, PTHR1
Gene Symbol
PTH1R
UniProt
Additional PTH1R/PTHR1 Products
Product Documents for Human PTH1R/PTHR1 Antibody
Product Specific Notices for Human PTH1R/PTHR1 Antibody
For research use only
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