Human PTH1R/PTHR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5709
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 734630
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PTH1R/PTHR1
Tyr23-Met189
Accession # Q03431
Tyr23-Met189
Accession # Q03431
Specificity
Detects human PTH1R/PTHR1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PTH1R/PTHR1 Antibody
Detection of Human PTH1R/PTHR1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos-2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB5709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTH1R/PTHR1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PTH1R/PTHR1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
2 µg/mL
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos‑2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, Saos‑2 human osteosarcoma cell line, and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTH1R/PTHR1
Long Name
Parathyroid Hormone Receptor 1
Alternate Names
PTHR, PTHR1
Gene Symbol
PTH1R
UniProt
Additional PTH1R/PTHR1 Products
Product Documents for Human PTH1R/PTHR1 Antibody
Product Specific Notices for Human PTH1R/PTHR1 Antibody
For research use only
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