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Human PXR/NR1I2 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H4417-00

R&D Systems, part of Bio-Techne
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PP-H4417-00

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Direct ELISA, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H4417

Product Specifications

Immunogen

Recombinant human PXR/NR1I2

Specificity

This antibody specifically recognizes human PXR1 and human PXR2. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human PXR/NR1I2 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 2 μg/mL with the appropriate secondary reagents to detect human PXR.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions and 3 μg/mL under non-reducing conditions with the appropriate secondary reagents to detect human PXR.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when stored at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PXR/NR1I2

Pregnane X Receptor (PXR; also known as SXR and NR1I2) is a 50 kDa member of the Nuclear Receptor (NR1) subfamily of the Nuclear Hormone Receptor family. It is a product of the PXR gene that gives rise to two basic isoforms; a 434 amino acid (aa) PXR1 form and 473 aa PXR2 form. PXR2 differs from PXR1 in that PXR2 contains a 39 aa N-terminal extension. Six PXR1 isoforms have been reported (Swiss Protein Accession # 075469). The anti-human PXR antibody recognizes all isoforms except the "B" isoforms which show a deletion of amino acids 1 - 55 (MEVRP...FNVM). PXR forms a heterodimer with Retinoid X Receptor (RXR) and binds pregnanolone.

Long Name

Pregnane X Receptor

Alternate Names

BXR, NR1I2, ONR1, PAR1, PAR2, SXR

Entrez Gene IDs

8856 (Human); 18171 (Mouse)

Gene Symbol

NR1I2

Additional PXR/NR1I2 Products

Product Documents for Human PXR/NR1I2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PXR/NR1I2 Antibody

For research use only

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