Human Rad17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1926
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 223802
Product Specifications
Immunogen
E. coli-derived recombinant human Rad17
Asn2-Arg240
Accession # NP_579922
Asn2-Arg240
Accession # NP_579922
Specificity
Detects human Rad17 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Rad17 Antibody
Immunoprecipitation of Human Rad17.
Rad17 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2-3 µg Mouse Anti-Human Rad17 Monoclonal Antibody (Catalog # MAB1926) for 1 hour on ice. Rad17-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Rad17 (left panel) was detected by Western blot using 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation.Applications for Human Rad17 Antibody
Application
Recommended Usage
Immunoprecipitation
2-3 µg/500 µg cell lysate
Sample: K562 human chronic myelogenous leukemia cell line, see our available Western blot detection antibodies
Sample: K562 human chronic myelogenous leukemia cell line, see our available Western blot detection antibodies
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad17
The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).
Alternate Names
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog
Gene Symbol
RAD17
UniProt
Additional Rad17 Products
Product Documents for Human Rad17 Antibody
Product Specific Notices for Human Rad17 Antibody
For research use only
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