Human Rad50 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4996

R&D Systems, part of Bio-Techne
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MAB4996
MAB4996-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 684945

View all Rad50 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rad50
Leu518-Leu881
Accession # Q92878

Specificity

Detects human Rad50 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Rad51 or rhRad53 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Rad50 Antibody

Detection of Human Rad50 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cells, HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Rad50 Monoclonal Antibody (Catalog # MAB4996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rad50 at approximately ~150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Rad50 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cells and HepG2 human hepatocellular carcinoma cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad50

Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes.  It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex.  This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells.  Human Rad50 is 1312 amino acids (aa) in length.  It has an apparent ATP binding site (aa 36-43) plus a coiled-coil region (aa 228-598) followed by a “zinc-hook” domain (aa 635-734) that mediates homodimerization.  There are multiple splice variants.  An alternate start site exists at Met140, there is a single Lys substitution for aa 723-1312, and three Lys substitute for aa 555-1312.  Over aa 518-881, human Rad50 is 96% aa identical to mouse Rad50.   

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Entrez Gene IDs

10111 (Human); 19360 (Mouse); 64012 (Rat)

Gene Symbol

RAD50

UniProt

Q92878

Additional Rad50 Products

Product Documents for Human Rad50 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rad50 Antibody

For research use only

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