Human/Rat Fgr Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3207
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769
Met1-Phe82
Accession # P09769
Specificity
Detects human and rat Fgr in Western blot.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Rat Fgr Antibody
Detection of Human/Rat Fgr by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Fgr Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3207) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Fgr at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Fgr in U937 Human Cell Line.
Fgr was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human/Rat Fgr Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3207) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Rat Fgr Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and L6 rat myoblast cell line
Sample: Raji human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fgr
Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.
Long Name
Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog
Alternate Names
Src2
Gene Symbol
FGR
UniProt
Additional Fgr Products
Product Documents for Human/Rat Fgr Antibody
Product Specific Notices for Human/Rat Fgr Antibody
For research use only
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